1 resultado para Genetic markers
em Publishing Network for Geoscientific
Filtro por publicador
- Aberdeen University (2)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (3)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (11)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (7)
- Aquatic Commons (18)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (5)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (3)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (6)
- Aston University Research Archive (1)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital | Sistema Integrado de Documentación | UNCuyo - UNCUYO. UNIVERSIDAD NACIONAL DE CUYO. (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (35)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (11)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (2)
- Bioline International (2)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (55)
- Brock University, Canada (4)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- CaltechTHESIS (1)
- CentAUR: Central Archive University of Reading - UK (35)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (74)
- Cochin University of Science & Technology (CUSAT), India (5)
- Collection Of Biostatistics Research Archive (2)
- CORA - Cork Open Research Archive - University College Cork - Ireland (1)
- Deakin Research Online - Australia (34)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Commons - Michigan Tech (3)
- Digital Commons at Florida International University (9)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (8)
- DigitalCommons@University of Nebraska - Lincoln (3)
- DRUM (Digital Repository at the University of Maryland) (1)
- Duke University (5)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (43)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Helda - Digital Repository of University of Helsinki (22)
- Indian Institute of Science - Bangalore - Índia (5)
- Infoteca EMBRAPA (2)
- Institutional Repository of Leibniz University Hannover (1)
- Instituto Superior de Psicologia Aplicada - Lisboa (1)
- National Center for Biotechnology Information - NCBI (34)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (1)
- Publishing Network for Geoscientific & Environmental Data (1)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (71)
- Queensland University of Technology - ePrints Archive (89)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (9)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório Científico do Instituto Politécnico de Santarém - Portugal (1)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositório do Centro Hospitalar de Lisboa Central, EPE - Centro Hospitalar de Lisboa Central, EPE, Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (4)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (193)
- SAPIENTIA - Universidade do Algarve - Portugal (10)
- Scientific Open-access Literature Archive and Repository (1)
- Universidad del Rosario, Colombia (2)
- Universidad Politécnica de Madrid (11)
- Universidade Complutense de Madrid (1)
- Universidade de Lisboa - Repositório Aberto (2)
- Universidade do Algarve (2)
- Universidade Federal do Pará (6)
- Universidade Federal do Rio Grande do Norte (UFRN) (5)
- Universidade Técnica de Lisboa (1)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (3)
- Université de Lausanne, Switzerland (11)
- Université de Montréal, Canada (6)
- Université Laval Mémoires et thèses électroniques (2)
- University of Queensland eSpace - Australia (56)
- WestminsterResearch - UK (1)
Resumo:
Pumas are one of the most studied terrestrial mammals because of their widespread distribution, substantial ecological impacts, and conflicts with humans. Extensive efforts, often employing genetic methods, are undertaken to manage this species. However, the comparison of population genetic data is difficult because few of the microsatellite loci chosen are shared across research programs. Here, we describe the development of PumaPlex, a high-throughput assay to genotype 25 single nucleotide polymorphisms in pumas. We validated PumaPlex in more than 700 North American pumas (Puma concolor couguar), and demonstrated its ability to generate reproducible genotypes and accurately identify individuals. Furthermore, we compared PumaPlex with traditional genotyping of 12 microsatellite loci in fecal DNA samples and found that PumaPlex produced significantly more genotypes with fewer false alleles. PumaPlex promotes the cross-laboratory comparison of genotypes, is easily expandable in the future, and is a valuable tool for the genetic monitoring and management of North American puma populations.